Ano
2015

Autores
Robl, D., dos Santos Costa, P., Büchli, F., da Silva Lima, D. J., da Silva Delabona, P., Squina, F. M., … & da Cruz Pradella, J. G.

Enhancing of sugar cane bagasse hydrolysis by Annulohypoxylon stygium glycohydrolases


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Referência Bibliográfica: Robl, D., dos Santos Costa, P., Büchli, F., da Silva Lima, D. J., da Silva Delabona, P., Squina, F. M., … & da Cruz Pradella, J. G. (2015). Enhancing of sugar cane bagasse hydrolysis by Annulohypoxylon stygium glycohydrolases. Bioresource technology, 177, 247-254.

Periódico: Bioresource Technology
DOI: 10.1016/j.biortech.2014.11.082

Resumo: The aim of this study was to develop a bioprocess for the production of β-glucosidase and pectinase from the fungus Annulohypoxylon stygium DR47. Media optimization and bioreactor cultivation using citrus bagasse and soybean bran were explored and revealed a maximum production of 6.26 U/mL of pectinase at pH 4.0 and 10.13 U/mL of β-glucosidase at pH 5.0. In addition, the enzymes extracts were able to replace partially Celluclast 1.5L in sugar cane bagasse hydrolysis. Proteomic analysis from A. stygiumcultures revealed accessory enzymes, mainly belong to the families GH3 and GH54, that would support enhancement of commercial cocktail saccharification yields. This is the first report describing bioreactor optimization for enzyme production from A. stygium with a view for more efficient degradation of sugar cane bagasse.