Bioethanol Production

Research Biography

He worked at Fermentation Lab of Butantan Institute (São Paulo – SP) as intern (2008-2009) in a project of improvement and scale-up of Streptococcus pneumoniae cellular vaccine, working with culture media improvement, fermentation process strategies and bioprocess scaling up criteria evaluation.

In April 2009 he joined as Process Engineer at Brazilian Bioethanol Science and Technology Laboratory (CTBE) of Brazilian Center for Research in Energy and Materials (CNPEM). He acted in the implementation of CTBE Pilot Plant and Bioprocess Laboratories, focused on specification, purchasing and installation projects of multipurpose equipments, mainly for use on second generation ethanol research and development projects.

Since 2010 he has been developing applied research in cellulolytic enzyme production, enzymatic hydrolysis and other bioprocesses.

Participation in technology development project in collaboration with multinational companies and financial support from BNDES, focused on development of second generation ethanol technology in lab and pilot scale (2012-now).

He also participates in research projects financed by Fapesp and CNPq focused on production and use of cellulolytic enzymes produced by filamentous fungi.


2004 – 2008

B.Sc. in Bioprocess and Biotechnology Engineering.
Federal University of Paraná – Curitiba-PR, Brazil.

Areas of Expertise
  1. Bioprocess Improvement
    Improvement of bioprocess conditions (culture media, process control parameters, impeller types and agitation intensity) focused on cellulolytic enzymes production by filamentous fungi;
  2. Enzymatic Hydrolysis
    Evaluation of lignocellulosic materials enzymatic hydrolysis. Studies with different enzymatic cocktails, agitation intensity, impeller type and process strategies;
  3. Bioprocess Scale-up
    Evaluation of scale-up criteria for bioprocesses, specially for cellulolytic enzymes production by filamentous fungi and enzymatic hydrolysis process.
Selected Publications and Patents

Selected Publications

  • José Geraldo da Cruz Pradella, Patricia dos Santos Costa, Priscila da Silva Delabona, Diogo Robl, Deise Juliana da Silva Lima and Daniel Kolling (2015). Modulation of Penicillium echinulatum glycoside hydrolases. Symposium on Biotechnology for Fuels and Chemicals, San Diego, CA.
  • José Geraldo da Cruz Pradella, Chanel Moacyr de Carli, Sarita C. Rabelo and Daniel Kolling (2015). Debottlenecking high-solids concentration enzymatic hydrolysis: ball-milling tumbling reactor and use of surfactants. Symposium on Biotechnology for Fuels and Chemicals, San Diego, CA.
  • Carolina Brás Costa, Priscila da Silva Delabona, Gisele Nunes Rodrigues, Daniel Kolling, Patricia dos Santos Costa, Deise Juliana da Silva Lima, Marcia da Silva Souza, Marcela Oliveira Lee, Alberdan Silva Santos and José Geraldo da Cruz Pradella (2015). Accessory enzymes produced by a newly isolated Lasiodiplodia theobromae from Amazon forest with potential for use in biomass degradation. Symposium on Biotechnology for Fuels and Chemicals, San Diego, CA.
  • MARTINS, L. F. ; KOLLING, D. ; CAMASSOLA, M. ; DILON, A. J. P. ; RAMOS, L. P (2008). Comparison between Penicillium echinulatum and Trichoderma reesei cellulases in relation to their activity against various cellulosic substrates. Bioresource Technology, v. 99, p. 1417-1424.
  • MARTINS, L. F.; KOLLING, D.; CAMASSOLA, M.; DILLON, A. J. P.; RAMOS, L. P. (2005). Perfil hidrolítico das celulases de Penicillium echinulatum. In: XV Simpósio Nacional de Bioprocessos, Recife – PE. Anais do XV Simpósio Nacional de Bioprocessos.